 James BaskettUncle Remus / Br'er Fox (voice) | Uncle Remus / Br'er Fox (voice) |
 Ruth WarrickSally | Sally |
 Bobby DriscollJohnny | Johnny |
 Luana PattenGinny | Ginny |
 Lucile WatsonGrandmother | Grandmother |
 Hattie McDanielAunt Tempy | Aunt Tempy |
 Erik RolfJohn | John |
 Glenn LeedyToby | Toby |
| MMary FieldMrs. Favers | Mrs. Favers |
| AAnita BrownMaid | Maid |
| GGeorgie NokesJake Favers | Jake Favers |
| GGene HollandJoe Flavers | Joe Flavers |
 Nick StewartBr'er Bear (voice) | Br'er Bear (voice) |
| JJohnny LeeBr'er Rabbit (voice) | Br'er Rabbit (voice) |
| HHelen CrozierMother Possum (voice) (uncredited) | Mother Possum (voice) (uncredited) |
| BBabette De CastroBirds (voice) (uncredited) | Birds (voice) (uncredited) |
| CCherie De CastroBirds (voice) (uncredited) | Birds (voice) (uncredited) |
| PPeggy De CastroBirds (voice) (uncredited) | Birds (voice) (uncredited) |
 Roy GlennBr'er Frog (voice) (uncredited) | Br'er Frog (voice) (uncredited) |
 Clarence NashMr. Bluebird (voice) (uncredited) | Mr. Bluebird (voice) (uncredited) |
| OOlivier UrbainMr. Favers (uncredited) | Mr. Favers (uncredited) |
 Ernestine WadeButterfly (uncredited) | Butterfly (uncredited) |
