The spirit of the evil Dr. Mabuse takes over the body of a famous professor. The professor/Dr. Mabuse then begins a new crime wave that terrorizes the city.
| Actor | Role |
|---|---|
 Peter van EyckMajor Bill Tern | Major Bill Tern |
 Dieter BorscheGeorge Cockstone | George Cockstone |
 Klaus KinskiInspector Joe Wright | Inspector Joe Wright |
 Sabine BethmannNancy Masterson | Nancy Masterson |
 Werner PetersInspektor Vulpius | Inspektor Vulpius |
 Wolfgang PreissGeist von Dr. Mabuse | Geist von Dr. Mabuse |
 Agnes WindeckGwendolyne Tern | Gwendolyne Tern |
 Hans NielsenChef vom Yard | Chef vom Yard |
 Albrecht SchoenhalsSir Robert Alingham | Sir Robert Alingham |
 Wolfgang LukschyErnest Hyliard | Ernest Hyliard |
 Ady BerberHenker | Henker |
| AAlbert BesslerKonservator | Konservator |
| RRuth WilbertPrinzessin Diana | Prinzessin Diana |
| AAnneliese WürtzRose | Rose |
 Sigurd LohdeBriefträger | Briefträger |
| AAlfred BraunNot listed | Not listed |
| GGert WiedenhofenKloppe | Kloppe |
 Walter RillaDr. Pohland / 'Dr. Mabuse' | Dr. Pohland / 'Dr. Mabuse' |
| JJürgen DraegerNot listed | Not listed |
 Michael GahrNot listed | Not listed |
| JJoachim NottkeRadio Announcer (voice) | Radio Announcer (voice) |