Velma discovers she's inherited her great-great-uncle's cursed castle in Transylvania, Pennsylvania. The team decide to go there on a spooky adventure.
| Actor | Role |
|---|---|
 Frank WelkerFred / Scooby-Doo (voice) | Fred / Scooby-Doo (voice) |
 Matthew LillardShaggy (voice) | Shaggy (voice) |
 Mindy CohnVelma (Voice) | Velma (Voice) |
 Grey DeLisleDaphne (voice) | Daphne (voice) |
 Dee Bradley BakerC.L. Magnus / Mr. Burger (voice) | C.L. Magnus / Mr. Burger (voice) |
 Diedrich BaderMrs. Vanders (voice) | Mrs. Vanders (voice) |
 Susanne BlakesleeTownswoman (voice) | Townswoman (voice) |
 Eric BauzaDaphanatic / Rock Dude (voice) | Daphanatic / Rock Dude (voice) |
 Jeff BennettIago / Schmidlap (voice) | Iago / Schmidlap (voice) |
 Kevin Michael RichardsonCuthbert Crawley / Inspector Krunch (voice) | Cuthbert Crawley / Inspector Krunch (voice) |
 Candi MiloGypsy / Lila (voice) | Gypsy / Lila (voice) |
 Corey BurtonBaron Basil (voice) | Baron Basil (voice) |
 Fred TatascioreFrankencreep (voice) | Frankencreep (voice) |
 John StephensonRoger (voice) (archive footage) | Roger (voice) (archive footage) |
 Casey KasemShaggy Rogers (voice) (archive footage) | Shaggy Rogers (voice) (archive footage) |