Scooby-Doo and the Mystery Inc. gang meet up with Batman and other friends to defeat evil villains and save the day.
| Actor | Role |
|---|---|
 Frank WelkerScooby-Doo / Fred Jones (voice) | Scooby-Doo / Fred Jones (voice) |
 Grey DeLisleDaphne Blake / Black Canary (voice) | Daphne Blake / Black Canary (voice) |
 Matthew LillardShaggy Rogers (voice) | Shaggy Rogers (voice) |
 Kate MicucciVelma Dinkley (voice) | Velma Dinkley (voice) |
 Diedrich BaderBatman (voice) | Batman (voice) |
 Jeff BennettJoker (voice) | Joker (voice) |
 Jeffrey CombsThe Question (voice) | The Question (voice) |
 John DiMaggioAquaman / Crimson Cloak (voice) | Aquaman / Crimson Cloak (voice) |
 Nika FuttermanCatwoman (voice) | Catwoman (voice) |
 Nicholas GuestMartian Manhunter (voice) | Martian Manhunter (voice) |
 John Michael HigginsThe Riddler (voice) | The Riddler (voice) |
 Tom KennyPenguin / Plastic Man (voice) | Penguin / Plastic Man (voice) |
 Kevin Michael RichardsonClayface / Detective Chimp (voice) | Clayface / Detective Chimp (voice) |
 Tara StrongHarley Quinn / Poison Ivy (voice) | Harley Quinn / Poison Ivy (voice) |
 Fred TatascioreHarvey Bullock (voice) | Harvey Bullock (voice) |