 Frank WelkerFred Jones / Scooby-Doo (voice) | Fred Jones / Scooby-Doo (voice) |
 Casey KasemShaggy Rogers (voice) | Shaggy Rogers (voice) |
 Heather NorthDaphne Blake (voice) | Daphne Blake (voice) |
 Nicole JaffeVelma Dinkley (voice) | Velma Dinkley (voice) |
 Candi MiloCharlene Otero / Museum Guide / Old Woman #1 (voice) | Charlene Otero / Museum Guide / Old Woman #1 (voice) |
 Maria Canals-BarreraSofia Otero / Old Woman #2 (voice) | Sofia Otero / Old Woman #2 (voice) |
 Jesse BorregoLuis Otero / Musician #2 / Suspicious Tourist (voice) | Luis Otero / Musician #2 / Suspicious Tourist (voice) |
| BBrandon GonzalesJorge Otero (voice) | Jorge Otero (voice) |
 Castulo GuerraSeñor Fuente / Man #1 (voice) | Señor Fuente / Man #1 (voice) |
 Benito MartinezEl Curandero / Man #2 (voice) | El Curandero / Man #2 (voice) |
| DDarlene MendozaNatalia Otero / Little Girl (voice) | Natalia Otero / Little Girl (voice) |
 Rita MorenoDoña Dolores / Woman #3 (voice) | Doña Dolores / Woman #3 (voice) |
| AAllyse RiveraCarolina Otero (voice) | Carolina Otero (voice) |
| CCasey SandinoSebastian Otero / Little Boy (voice) | Sebastian Otero / Little Boy (voice) |
 Eddie SantiagoAlejo Otero / Musician #1 (voice) | Alejo Otero / Musician #1 (voice) |
 Rip TaylorMr. Smiley / Ghost of Señor Otero (voice) | Mr. Smiley / Ghost of Señor Otero (voice) |
 Michael ForestEl Chuprecabra (voice) | El Chuprecabra (voice) |
