In the year 1882, Sanshirou comes to Tokyo seeking for a worthy martial arts teacher. There, he meets Yano - a judo master who single-handedly defeats 39 jujitsu practitioners. Extremely impressed by his skill, Sanshirou decides to become his disciple. Later, Sanshirou falls for Sayo Murai - a girl whose father is a martial arts instructor at the local police department; and he also has to face Higaki Gennosuke, a skilled jujitsu fighter and his greatest rival.
| Actor | Role |
|---|---|
 Yoshimi IwasakiOtomi Murai (voice) | Otomi Murai (voice) |
 Hideki SaijoSanshiro Sugata (voice) | Sanshiro Sugata (voice) |
 Chikao OhtsukaGennosuke Higaki (voice) | Gennosuke Higaki (voice) |
 Nachi NozawaShogoro Yano (voice) | Shogoro Yano (voice) |
 Kei TomiyamaKinnosuke Natsume (voice) | Kinnosuke Natsume (voice) |
| YYouichi MiyagawaHansuke Murai (voice) | Hansuke Murai (voice) |
 Osamu KatoSaburo Monma (voice) | Saburo Monma (voice) |
 Rihoko YoshidaTakako (voice) | Takako (voice) |
 Enjou SanyuuteiNarrator (voice) | Narrator (voice) |
 Hiroko MaruyamaNot listed | Not listed |
 Kaneta KimotsukiNot listed | Not listed |
 Kenichi OgataNot listed | Not listed |
 Koichi KitamuraNot listed | Not listed |
 Reizō NomotoNot listed | Not listed |
 Shigezo SasaokaNot listed | Not listed |
 Keaton YamadaNot listed | Not listed |
| TToku NishioNot listed | Not listed |
 Yoku ShioyaNot listed | Not listed |
 Daisuke GoriNot listed | Not listed |
 Yusaku YaraNot listed | Not listed |