Gene and Frog arrive with a herd of horses for Gene'e brother, a diamond prospector whose work has attracted the interest of a bunch of badguys.
| Actor | Role |
|---|---|
 Gene AutryGene Autry | Gene Autry |
 Smiley BurnetteFrog Millhouse | Frog Millhouse |
 Maxine DoyleGwen Barkley | Gwen Barkley |
 ChampionChamp | Champ |
 LeRoy MasonJohn Cardigan | John Cardigan |
 Earle HodginsBarkey McCusky | Barkey McCusky |
 Dick WesselHenchman Craig Johnson | Henchman Craig Johnson |
| BBuddy WilliamsChief Bosuto | Chief Bosuto |
| EElmer FainChief Bosuto's Son | Chief Bosuto's Son |
| CCorny AndersonNamba (as Cornie Anderson) | Namba (as Cornie Anderson) |
| FFrankie MarvinCowboy / Soldier | Cowboy / Soldier |
| KKen CooperTex Autry | Tex Autry |