 Seth GreenThe Nerd / Batman / Robin / Aquaman / The Penguin / Martian Manhunter / Toyman / Scarecrow / Gorilla Grodd / Little Cheese / Swamp Thing / Abin Sur / Ganthet / Green Arrow / Hawkman / Jimmy Olsen / Various (voice) | The Nerd / Batman / Robin / Aquaman / The Penguin / Martian Manhunter / Toyman / Scarecrow / Gorilla Grodd / Little Cheese / Swamp Thing / Abin Sur / Ganthet / Green Arrow / Hawkman / Jimmy Olsen / Various (voice) |
 Breckin MeyerSuperman / Mirror Master (voice) | Superman / Mirror Master (voice) |
 Nathan FillionGreen Lantern / Mr. Freeze (voice) | Green Lantern / Mr. Freeze (voice) |
 Alex BorsteinWonder Woman / Giganta / Woman (voice) | Wonder Woman / Giganta / Woman (voice) |
 Matthew SenreichThe Flash / Chillblaine / Brainiac / Wildcat (voice) | The Flash / Chillblaine / Brainiac / Wildcat (voice) |
 Alfred MolinaLex Luthor / Firestorm / Mr. Banjo (voice) | Lex Luthor / Firestorm / Mr. Banjo (voice) |
 Paul ReubensThe Riddler (voice) | The Riddler (voice) |
 Neil Patrick HarrisTwo-Face / Black Manta (voice) | Two-Face / Black Manta (voice) |
 Kevin ShinickNarrator / Captain Cold (voice) | Narrator / Captain Cold (voice) |
 Abraham BenrubiCyborg/ Kilowog / Appa Ali Apsa / Solomon Grundy (voice) | Cyborg/ Kilowog / Appa Ali Apsa / Solomon Grundy (voice) |
 Zeb WellsSinestro / Swamp Thing (voice) | Sinestro / Swamp Thing (voice) |
 Megan FoxLois Lane (voice) | Lois Lane (voice) |
 Aaron PaulGlenn (voice) | Glenn (voice) |
 Tara StrongHarley Quinn / Selena Gomez (voice) | Harley Quinn / Selena Gomez (voice) |
| TTom RootIcicle/B'dg (voice) | Icicle/B'dg (voice) |
