 Charles LaughtonRembrandt van Rijn | Rembrandt van Rijn |
 Gertrude LawrenceGeertje Dirx | Geertje Dirx |
 Elsa LanchesterHendrickje Stoffels | Hendrickje Stoffels |
 Edward ChapmanFabrizius | Fabrizius |
 Walter HuddCapt. Banning Cocq | Capt. Banning Cocq |
 Roger LiveseyBeggar Saul | Beggar Saul |
| JJohn BryningTitus van Rijn | Titus van Rijn |
| SSam LiveseyAuctioneer | Auctioneer |
 Herbert LomasHarmenzs van Rijn | Harmenzs van Rijn |
 Allan JeayesDr. Tulp | Dr. Tulp |
 John ClementsGovaert Flinck | Govaert Flinck |
 Raymond HuntleyLudwick | Ludwick |
 Abraham SofaerDr. Menasseh | Dr. Menasseh |
 Laurence HanrayHeertsbeeke | Heertsbeeke |
 Austin TrevorMarquis de Grand Coeur | Marquis de Grand Coeur |
 Henry HewittJan Six | Jan Six |
 Gertrude MusgroveAgelintje | Agelintje |
| RRichard GofeTitus (child) | Titus (child) |
 Basil GillAdrien van Rijn | Adrien van Rijn |
| BBarry LiveseyPeasant Lad | Peasant Lad |
| JJames CarneyPeasant Lad | Peasant Lad |
 Jack LiveseyJourneyman | Journeyman |
 John TurnbullMinister | Minister |
 Edmund WillardVan Zeeland | Van Zeeland |
| HHector AbbasBurgher at Auction (uncredited) | Burgher at Auction (uncredited) |
 Evelyn AnkersParty Girl (uncredited) | Party Girl (uncredited) |
| BBaroness BaranyCriada (uncredited) | Criada (uncredited) |
| LLewis BroughtonSaskia’s Brother (uncredited) | Saskia’s Brother (uncredited) |
| AArthur BurneUndetermined Secondary Rôle (uncredited) | Undetermined Secondary Rôle (uncredited) |
| RRobin BurnsTavern Patron (uncredited) | Tavern Patron (uncredited) |
