Deputies Gene Autry and Frog go up against modern cattle rustlers. These rustlers use technology such as, airplanes, radios and refrigerated trucks to steal the cows, butcher them in the field and ship them out before getting caught. This causes the town to bring in a modern NYC detective to catch the crooks, but will Autry and Frog be permanently out of a job?
| Actor | Role |
|---|---|
 Gene AutryHelen Morgan | Helen Morgan |
 Smiley BurnetteHelen Morgan | Helen Morgan |
 Ann RutherfordHelen Morgan | Helen Morgan |
 William FarnumSheriff Matt Doniphon | Sheriff Matt Doniphon |
 Arthur LoftJack Shannon | Jack Shannon |
| FFrankie MarvinDeputy Stubby | Deputy Stubby |
 House Peters Jr.Jim Shannon | Jim Shannon |
 James C. MortonEustace P. Quackenbush | Eustace P. Quackenbush |
| MMaston WilliamsThad Slaughter | Thad Slaughter |
 Frank LaRueThe Judge (as Frank La Rue) | The Judge (as Frank La Rue) |
 Milburn MoranteEzra | Ezra |