 Yo OizumiProfessor Layton (voice) | Professor Layton (voice) |
 Maki HorikitaLuke Triton (voice) | Luke Triton (voice) |
 Saki AibuEmmy Altava (voice) | Emmy Altava (voice) |
 Nana MizukiJanice Quatlane (voice) | Janice Quatlane (voice) |
 Atsuro WatabeJean Descole (voice) | Jean Descole (voice) |
 Hochu OtsukaInspector Clamp Grosky (voice) | Inspector Clamp Grosky (voice) |
 Iemasa KayumiOswald Whistler (voice) | Oswald Whistler (voice) |
 Fumiko OrikasaMelina Whistler (voice) | Melina Whistler (voice) |
 Kikuko InoueCelia Raidley (voice) | Celia Raidley (voice) |
 Sumire MorohoshiNina (voice) | Nina (voice) |
 Shozo IizukaCurtis O'Donnell (voice) | Curtis O'Donnell (voice) |
 Kenta MiyakeMarco Brock (voice) | Marco Brock (voice) |
 Megumi ToyoguchiAmelia Ruth (voice) | Amelia Ruth (voice) |
 Jouji NakataFrederick Bargland (voice) | Frederick Bargland (voice) |
 LiLiCoAnnie Dretche (voice) | Annie Dretche (voice) |
 Koichi YamaderaPierre Starbuck (voice) | Pierre Starbuck (voice) |
 Minoru InabaDon Paolo (voice) | Don Paolo (voice) |
 Shirou SaitouInspector Chelmey (voice) | Inspector Chelmey (voice) |
 Mamiko NotoFlora Reinhold (voice) | Flora Reinhold (voice) |
 Rokuro NayaDr. Andrew Schrader (voice) | Dr. Andrew Schrader (voice) |
