 James CordenPeter Rabbit (voice) | Peter Rabbit (voice) |
 Rose ByrneBea | Bea |
 Domhnall GleesonMr. Thomas McGregor | Mr. Thomas McGregor |
 David OyelowoNigel Basil-Jones | Nigel Basil-Jones |
 Margot RobbieFlopsy Rabbit / The Narrator (voice) | Flopsy Rabbit / The Narrator (voice) |
 Elizabeth DebickiMopsy Rabbit (voice) | Mopsy Rabbit (voice) |
 Aimee HorneCottontail Rabbit (voice) | Cottontail Rabbit (voice) |
 Colin MoodyBenjamin Bunny (voice) | Benjamin Bunny (voice) |
 Lennie JamesBarnabas (voice) | Barnabas (voice) |
 Damon HerrimanTom Kitten (voice) | Tom Kitten (voice) |
 Hayley AtwellMittens (voice) | Mittens (voice) |
 Rupert DegasSamuel Whiskers / Little Pig Robinson (voice) | Samuel Whiskers / Little Pig Robinson (voice) |
 SiaMrs. Tiggy-Winkle (voice) | Mrs. Tiggy-Winkle (voice) |
 Sam NeillTommy Brock (voice) | Tommy Brock (voice) |
 Ewen LesliePigling Bland (voice) | Pigling Bland (voice) |
 David WenhamJohnny Town-Mouse (voice) | Johnny Town-Mouse (voice) |
| WWill ReicheltJW Rooster II (voice) | JW Rooster II (voice) |
| CChristian GazalFelix D'eer (voice) | Felix D'eer (voice) |
 Tim MinchinBusker K. Bushy, Esq (voice) | Busker K. Bushy, Esq (voice) |
| SStewart AlvesMr. Tod (voice) | Mr. Tod (voice) |
| FFaizan Munawar VaryaRay Rabbit (voice) | Ray Rabbit (voice) |
 Tara MoriceOfficiant | Officiant |
 Dave LawsonChris | Chris |
 Alex BliasPhil | Phil |
 Jude HylandToy Shop Young Boy | Toy Shop Young Boy |
| NNeilesh AmbuPasserby (uncredited) | Passerby (uncredited) |
| TTom GoldingCarlos (Aide) | Carlos (Aide) |
