 James CordenPeter Rabbit (voice) | Peter Rabbit (voice) |
 Rose ByrneBeatrix 'Bea' Potter / Jemima Puddle-Duck (voice) | Beatrix 'Bea' Potter / Jemima Puddle-Duck (voice) |
 Domhnall GleesonThomas McGregor / Mr. Jeremy Fisher (voice) | Thomas McGregor / Mr. Jeremy Fisher (voice) |
 Margot RobbieFlopsy Rabbit / Narrator (voice) | Flopsy Rabbit / Narrator (voice) |
 Elizabeth DebickiMopsy Rabbit (voice) | Mopsy Rabbit (voice) |
 Daisy RidleyCottontail Rabbit (voice) | Cottontail Rabbit (voice) |
 Colin MoodyBenjamin Bunny (voice) | Benjamin Bunny (voice) |
 Sam NeillMr. McGregor / Tommy Brock (voice) | Mr. McGregor / Tommy Brock (voice) |
 Marianne Jean-BaptisteHarrods General Manager | Harrods General Manager |
 Felix WilliamsonDerek | Derek |
 SiaMrs. Tiggy-Winkle (voice) | Mrs. Tiggy-Winkle (voice) |
 Fayssal BazziMr. Tod (voice) | Mr. Tod (voice) |
 Ewen LesliePigling Bland (voice) | Pigling Bland (voice) |
| CChristian GazalFelix D'eer (voice) | Felix D'eer (voice) |
 Rachel WardJosephine Rabbit (voice) | Josephine Rabbit (voice) |
 Bryan BrownMr. Rabbit (voice) | Mr. Rabbit (voice) |
 David WenhamJohnny Town-Mouse (voice) | Johnny Town-Mouse (voice) |
| WWill ReicheltJW Rooster II (voice) | JW Rooster II (voice) |
 Ming-Zhu HiiSarabeth | Sarabeth |
| LLance KerfuffleJ. David Scarfington | J. David Scarfington |
 Natalie DewJanelle | Janelle |
| TTerenia EdwardsSiobhan | Siobhan |
 Jermaine LiburdHarrods Security | Harrods Security |
| TTom GreavesHarrods Security | Harrods Security |
 Sacha HorlerBetty | Betty |
 Alex BliasPhil | Phil |
 Dave LawsonChris | Chris |
 Sam HaftPostman | Postman |
 Jessica FriedmanSinging Sparrow (voice) | Singing Sparrow (voice) |
| SShana HalliganSinging Sparrow (voice) | Singing Sparrow (voice) |
