The eccentric and childish Pee-wee Herman embarks on a big adventure when his beloved bicycle is stolen. Armed with information from a fortune-teller and a relentless obsession with his prized possession, Pee-wee encounters a host of odd characters and bizarre situations as he treks across the country to recover his bike.
| Actor | Role |
|---|---|
 Paul ReubensPee-wee Herman | Pee-wee Herman |
 E. G. DailyDottie | Dottie |
 Mark HoltonFrancis | Francis |
 Diane SalingerSimone | Simone |
 Judd OmenMickey | Mickey |
 Irving HellmanMr. Crowtrey | Mr. Crowtrey |
 Monte LandisMario | Mario |
 Damon MartinChip | Chip |
 Alice NunnLarge Marge | Large Marge |
 David C. GlasserBMX Kid | BMX Kid |
 Gregory BrownBMX Kid | BMX Kid |
 Daryl Keith RoachChuck | Chuck |
 Bill CablePoliceman #1 | Policeman #1 |
 Peter LooneyPoliceman #2 | Policeman #2 |
 Starletta DuPoisSgt. Hunter | Sgt. Hunter |
 Professor Toru TanakaButler | Butler |
 Ed HerlihyMr. Buxton | Mr. Buxton |
 Ralph SeymourFrancis' Accomplice | Francis' Accomplice |
 Lou CutellAmazing Larry | Amazing Larry |
 Raymond MartinoGang Member | Gang Member |
 Erica YohnMadam Ruby | Madam Ruby |
| BBill W. RichmondHighway Patrolman | Highway Patrolman |
| EEd GriffithTrucker | Trucker |
 Simmy BowMan in Diner | Man in Diner |
 Jon HarrisAndy | Andy |
 Carmen FilpiHobo Jack | Hobo Jack |
 Jan HooksTina | Tina |
| JJohn MoodyBus Clerk | Bus Clerk |
| JJohn O'NeillCowboy #1 | Cowboy #1 |
| AAlex SharpCowboy #2 | Cowboy #2 |