The twelfth night of New Japan Cup 2021 took place on March 20, 2021, at Xebio Arena Sendai in Sendai, Miyagi, Japan, and featured two semifinal matches.
| Actor | Role |
|---|---|
 Shin TakagiShingo Takagi | Shingo Takagi |
 Takaaki WatanabeEVIL | EVIL |
 David Stephen Finlay IIIDavid Finlay | David Finlay |
 William OspreayWill Ospreay | Will Ospreay |
 Hiroshi TanahashiHiroshi Tanahashi | Hiroshi Tanahashi |
 Kazuchika OkadaKazuchika Okada | Kazuchika Okada |
 Kota IbushiKota Ibushi | Kota Ibushi |
 Jamie WhiteJay White | Jay White |
 Kenta KobayashiKENTA | KENTA |
 Yujiro TakahashiYujiro Takahashi | Yujiro Takahashi |
 Tatsuya HayamaDOUKI | DOUKI |
 Taichiro MakiTaichi | Taichi |
 Luke James Uggles EatwellZack Sabre Jr. | Zack Sabre Jr. |
 Tetsuya ShimizuBUSHI | BUSHI |
 Seiya SanadaSANADA | SANADA |
 Tetsuya NaitoTetsuya Naito | Tetsuya Naito |
 Sho TanakaSHO | SHO |
 Toru YanoToru Yano | Toru Yano |
 Nobuo YoshihashiYOSHI-HASHI | YOSHI-HASHI |
 Fale SimitaitokoBad Luck Fale | Bad Luck Fale |
 Steven OwensChase Owens | Chase Owens |
 Shoji AkiyoshiJado | Jado |
 Joseph RobinsonJuice Robinson | Juice Robinson |
 Yuya UemuraYuya Uemura | Yuya Uemura |
 Tomoyuki OkaGreat-O-Khan | Great-O-Khan |
 Jeffrey CobbJeff Cobb | Jeff Cobb |