Chaos reigns at the natural history museum when night watchman Larry Daley accidentally stirs up an ancient curse, awakening Attila the Hun, an army of gladiators, a Tyrannosaurus rex and other exhibits.
| Actor | Role |
|---|---|
 Ben StillerLarry Daley | Larry Daley |
 Carla GuginoRebecca | Rebecca |
 Dick Van DykeCecil | Cecil |
 Mickey RooneyGus | Gus |
 Bill CobbsReginald | Reginald |
 Jake CherryNick Daley | Nick Daley |
 Ricky GervaisDr. McPhee | Dr. McPhee |
 Robin WilliamsTheodore Roosevelt | Theodore Roosevelt |
 Kim RaverErica Daley | Erica Daley |
 Patrick GallagherAttila the Hun | Attila the Hun |
 Rami MalekAhkmenrah | Ahkmenrah |
 Pierfrancesco FavinoChristopher Columbus | Christopher Columbus |
 Charlie MurphyTaxi Driver | Taxi Driver |
 Steve CooganOctavius | Octavius |
 Mizuo PeckSacajawea | Sacajawea |
 Kerry van der GriendNeanderthal #1 | Neanderthal #1 |
 Dan RizzutoNeanderthal #2 | Neanderthal #2 |
 Matthew HarrisonNeanderthal #3 | Neanderthal #3 |
 Jody RacicotNeanderthal #4 | Neanderthal #4 |
 Paul RuddDon | Don |
 Anne MearaDebbie | Debbie |
 Martin ChristopherLewis | Lewis |
 Martin SimsClark | Clark |
 Randy LeeHun #1 | Hun #1 |
 Darryl QuonHun #2 | Hun #2 |
 Gerald WongHun #3 | Hun #3 |
 Paul ChengHun #4 | Hun #4 |
 Teagle F. BougereTeacher (Mike) | Teacher (Mike) |
 Pat KiernanTV News Anchor | TV News Anchor |
 Nico McEownFriend #1 | Friend #1 |