On May 30, 2005, on a high school graduation trip to Aruba, she disappeared without a trace.
| Actor | Role |
|---|---|
 Amy GumenickNatalee Holloway | Natalee Holloway |
 Tracy PollanBeth Holloway-Twitty | Beth Holloway-Twitty |
 Grant ShowJug Twitty | Jug Twitty |
 Catherine DentCarol | Carol |
 Natasha LoringHayley | Hayley |
| JJacques StrydomJoran Van Der Sloot | Joran Van Der Sloot |
| KKim SenoglesJustine | Justine |
| EErin CloytierDarla | Darla |
 Clayton EvertsonDeepak Kalpoe | Deepak Kalpoe |
 Hein De VriesSatish Kalpoe | Satish Kalpoe |
 Julie SummersBarbara Waxman | Barbara Waxman |
 Cokey FalkowPatrick van Eem | Patrick van Eem |
| LLeigh BremridgeDiana Peterson | Diana Peterson |
 Susan DanfordMaggie | Maggie |
 Sean Cameron MichaelPaul van der Sloot | Paul van der Sloot |
 Sean HiggsDetective Frank Sneider | Detective Frank Sneider |
 André JacobsDetective Roemer | Detective Roemer |
 Bjorn SteinbachBrent | Brent |
 Wayne HarrisonDave Holloway | Dave Holloway |