 Tara StrongTwilight Sparkle (voice) | Twilight Sparkle (voice) |
 Ashleigh BallApplejack / Rainbow Dash (voice) | Applejack / Rainbow Dash (voice) |
 Andrea LibmanPinkie Pie / Fluttershy (voice) | Pinkie Pie / Fluttershy (voice) |
 Tabitha St. GermainRarity / Princess Luna / Granny Smith / Derpy Hooves (voice) | Rarity / Princess Luna / Granny Smith / Derpy Hooves (voice) |
 Cathy WeseluckSpike (voice) | Spike (voice) |
 Emily BluntTempest Shadow / Fizzlepop Berrytwist (voice) | Tempest Shadow / Fizzlepop Berrytwist (voice) |
 Michael PeñaGrubber (voice) | Grubber (voice) |
 Liev SchreiberThe Storm King (voice) | The Storm King (voice) |
 Taye DiggsCapper (voice) | Capper (voice) |
 Zoe SaldañaCaptain Celaeno (voice) | Captain Celaeno (voice) |
 Kristin ChenowethPrincess Skystar (voice) | Princess Skystar (voice) |
 Uzo AdubaQueen Novo (voice) | Queen Novo (voice) |
 SiaSongbird Serenade (voice) | Songbird Serenade (voice) |
 Rebecca ShoichetTwilight Sparkle (singing voice) | Twilight Sparkle (singing voice) |
 Shannon Chan-KentPinkie Pie (singing voice) | Pinkie Pie (singing voice) |
 Kazumi EvansRarity (singing voice) | Rarity (singing voice) |
 Mark OliverFirst Mate Mullet (voice) | First Mate Mullet (voice) |
 Max MartiniBoyle (voice) | Boyle (voice) |
| AAdam BengisCode Red (voice) | Code Red (voice) |
 Nicole OliverPrincess Celestia / Lix Spittle (voice) | Princess Celestia / Lix Spittle (voice) |
 Britt McKillipPrincess Cadance (voice) | Princess Cadance (voice) |
 Brian DobsonVerko (voice) | Verko (voice) |
 Peter NewBig McIntosh (voice) | Big McIntosh (voice) |
 Michelle CreberApple Bloom (voice) | Apple Bloom (voice) |
 Michael DobsonBulk Biceps (voice) | Bulk Biceps (voice) |
 Sam VincentParty Favor (voice) | Party Favor (voice) |
