When Twilight Sparkle and her friends arrive in Hope Hollow, they find the town and all its residents completely devoid of color their dream vacation is starting to feel more like a nightmare. The Mane 6 will have to solve the mystery behind this washed-out world if they hope to bring the 'rainbow' back to the Rainbow Festival and color back to the lives of their new friends.
| Actor | Role |
|---|---|
 Ashleigh BallApplejack / Rainbow Dash (voice) | Applejack / Rainbow Dash (voice) |
 Andrea LibmanFluttershy / Pinkie Pie (voice) | Fluttershy / Pinkie Pie (voice) |
 Tabitha St. GermainRarity (voice) | Rarity (voice) |
 Tara StrongTwilight Sparkle (voice) | Twilight Sparkle (voice) |
 Cathy WeseluckSpike (voice) | Spike (voice) |
 Ian HanlinMayor Sunny Skies / Pony #1 / Man Pony / Boy Pony (voice) | Mayor Sunny Skies / Pony #1 / Man Pony / Boy Pony (voice) |
 Kelly MetzgerPetunia Petals / Customer #1 (voice) | Petunia Petals / Customer #1 (voice) |
| RRhona RheesTorque Wrench / Woman Pony / Girl Pony (voice) | Torque Wrench / Woman Pony / Girl Pony (voice) |
 Racquel BelmonteKerfuffle (voice) | Kerfuffle (voice) |
 Michael DaingerfieldMr. Hoofington (voice) | Mr. Hoofington (voice) |
| TTerry KlassenMoody Root (voice) | Moody Root (voice) |
 Veena SoodMrs. Hoofington (voice) | Mrs. Hoofington (voice) |
 Sabrina PitreBarley Barrel (voice) | Barley Barrel (voice) |
 David A. KayePickle Barrel (voice) | Pickle Barrel (voice) |