 Reese WitherspoonSusan Murphy / Ginormica (voice) | Susan Murphy / Ginormica (voice) |
 Seth RogenB.O.B. (voice) | B.O.B. (voice) |
 Hugh LaurieDr. Cockroach Ph.D. (voice) | Dr. Cockroach Ph.D. (voice) |
 Rainn WilsonGallaxhar (voice) | Gallaxhar (voice) |
 Will ArnettThe Missing Link (voice) | The Missing Link (voice) |
 Kiefer SutherlandGeneral W.R. Monger (voice) | General W.R. Monger (voice) |
 Paul RuddDerek Dietl (voice) | Derek Dietl (voice) |
 Stephen ColbertPresident Hathaway (voice) | President Hathaway (voice) |
 Julie WhiteWendy Murphy (voice) | Wendy Murphy (voice) |
 Jeffrey TamborCarl Murphy (voice) | Carl Murphy (voice) |
 Amy PoehlerComputer (voice) | Computer (voice) |
 Ed HelmsNews Reporter (voice) | News Reporter (voice) |
 Renée ZellwegerKatie (voice) | Katie (voice) |
 Sean BishopPrivate Bullhorn / Helicopter Pilot / Advisor Ortega (voice) | Private Bullhorn / Helicopter Pilot / Advisor Ortega (voice) |
 Rich DietlCommander / Advisor Smith (voice) | Commander / Advisor Smith (voice) |
 Rob LettermanSecret Service Man #1 / Lieutenant (voice) | Secret Service Man #1 / Lieutenant (voice) |
 Tom McGrathWilson (voice) | Wilson (voice) |
 Chris MillerAdvisor Cole / Army Commander Jones (voice) | Advisor Cole / Army Commander Jones (voice) |
 Mike MitchellAdvisor Wedgie (voice) | Advisor Wedgie (voice) |
 Kent OsborneTechnician Jerry (voice) | Technician Jerry (voice) |
 Latifa OuaouAdvisor Ouaou / Mrs. Ronson / Female Advisor #2 / Panicked Guest (voice) | Advisor Ouaou / Mrs. Ronson / Female Advisor #2 / Panicked Guest (voice) |
 Geoffrey PomeroySoldier #4 / Pilot / Soldier #3 / News Technician (voice) | Soldier #4 / Pilot / Soldier #3 / News Technician (voice) |
 David P. SmithSoldier #1 / Advisor Jackson (voice) | Soldier #1 / Advisor Jackson (voice) |
 Lisa StewartBridesmaid Candy (voice) | Bridesmaid Candy (voice) |
 Conrad VernonAdvisor Hawk / Advisor Dither / Minister / Secret Service Man #2 / Mama Dietl (voice) | Advisor Hawk / Advisor Dither / Minister / Secret Service Man #2 / Mama Dietl (voice) |
 John KrasinskiCuthbert (voice) | Cuthbert (voice) |
 Stephen KearinTechnician Bee (voice) | Technician Bee (voice) |
