 Sandra BullockScarlet Overkill (voice) | Scarlet Overkill (voice) |
 Jon HammHerb Overkill (voice) | Herb Overkill (voice) |
 Michael KeatonWalter Nelson (voice) | Walter Nelson (voice) |
 Allison JanneyMadge Nelson (voice) | Madge Nelson (voice) |
 Steve CooganProfessor Flux / Tower Guard (voice) | Professor Flux / Tower Guard (voice) |
 Jennifer SaundersReine Elizabeth II (voice) | Reine Elizabeth II (voice) |
 Geoffrey RushNarrator (voice) | Narrator (voice) |
 Steve CarellYoung Gru (voice) | Young Gru (voice) |
 Pierre CoffinThe Minions (voice) | The Minions (voice) |
 Katy MixonTina (voice) | Tina (voice) |
 Michael BeattieVNC Announcer / Walter Jr. (voice) | VNC Announcer / Walter Jr. (voice) |
 Hiroyuki SanadaSumo Villain (voice) | Sumo Villain (voice) |
 Dave RosenbaumFabrice (voice) | Fabrice (voice) |
| AAlex DowdingRoyal Advisor (voice) | Royal Advisor (voice) |
 Paul ThornleyNews Reporter (voice) | News Reporter (voice) |
 Kyle BaldaAdditional Voices (voice) | Additional Voices (voice) |
 Ava AcresAdditional Voices (voice) | Additional Voices (voice) |
 Sherry LynnAdditional Voices (voice) | Additional Voices (voice) |
 Mickie McGowanAdditional Voices (voice) | Additional Voices (voice) |
 Carlos AlazraquiAdditional Voices (voice) | Additional Voices (voice) |
 Lori AlanAdditional Voices (voice) | Additional Voices (voice) |
| DDaniel BarkerAdditional Voices (voice) | Additional Voices (voice) |
 Bob BergenAdditional Voices (voice) | Additional Voices (voice) |
 Jim CummingsAdditional Voices (voice) | Additional Voices (voice) |
 John CyganAdditional Voices (voice) | Additional Voices (voice) |
 Brian T. DelaneyAdditional Voices (voice) | Additional Voices (voice) |
 Bill FarmerAdditional Voices (voice) | Additional Voices (voice) |
 Keith FergusonAdditional Voices (voice) | Additional Voices (voice) |
 Jess HarnellAdditional Voices (voice) | Additional Voices (voice) |
| EEve KarpfAdditional Voices (voice) | Additional Voices (voice) |
