 Magda VášáryováMary Stuart | Mary Stuart |
| JJoan SutherlandMary Stuart (singing voice) | Mary Stuart (singing voice) |
 Kamila MagálováQueen Elizabeth I | Queen Elizabeth I |
| HHuguette TourangeauQueen Elizabeth I (singing voice) | Queen Elizabeth I (singing voice) |
 Milan KňažkoEarl of Leicester | Earl of Leicester |
 Luciano PavarottiEarl of Leicester (singing voice) | Earl of Leicester (singing voice) |
| PPřemysl KočíGeorge Talbot | George Talbot |
 Roger SoyerGeorge Talbot (singing voice) | George Talbot (singing voice) |
 Jan PřeučilWilliam Cecil | William Cecil |
 James MorrisWilliam Cecil (singing voice) | William Cecil (singing voice) |
 Jiřina JiráskováHanna Kennedy | Hanna Kennedy |
| MMargreta ElkinsHanna Kennedy (singing voice) | Hanna Kennedy (singing voice) |
 Luděk MunzarAmias Paulet | Amias Paulet |
 Vladimír DlouhýMortimer | Mortimer |
 Karel RodenWilliam Davison | William Davison |
 Jiří ValaMelville | Melville |
| ZZdeněk OrnestKent (uncredited) | Kent (uncredited) |
 Kornelia BojeMary Stuart (voice) | Mary Stuart (voice) |
| CChrista BerndlQueen Elizabeth I (voice) | Queen Elizabeth I (voice) |
| RRandolf KronbergEarl of Leicester (voice) | Earl of Leicester (voice) |
| BBettina SchönHanna Kennedy (voice) | Hanna Kennedy (voice) |
 Hartmut ReckAmias Paulet (voice) | Amias Paulet (voice) |
 Martin UmbachMortimer (voice) | Mortimer (voice) |
 Michael CramerMelville (voice) | Melville (voice) |