| MMisty JonesAccused Mother | Accused Mother |
| JJane AlexanderAccused Mother | Accused Mother |
| JJulie PatrickAccused Mother | Accused Mother |
| RRonald JonesMisty's Husband | Misty's Husband |
| BBrian MorganInvestigative Reporter | Investigative Reporter |
| MMarc FeldmanPsychiatrist - University of Alabama, Birmingham | Psychiatrist - University of Alabama, Birmingham |
| MMark PatrickJulie's Husband | Julie's Husband |
| EEllen ThompsonGrandmother | Grandmother |
| PPenny MellorActivist for Accused Mothers | Activist for Accused Mothers |
| DDavid SouthallProfessor of Pediatrics (as Dr. David Southall) | Professor of Pediatrics (as Dr. David Southall) |
| TTom RyanMedical Malpractice Lawyer | Medical Malpractice Lawyer |
| CColin MorelyDirector of Neonatal Medicine | Director of Neonatal Medicine |
| RRoy MeadowProfessor of Pediatrics (as Sir Roy Meadow) | Professor of Pediatrics (as Sir Roy Meadow) |
| EEugene GreesonMisty's Grandfather (as Eugene 'Papaw' Greeson) | Misty's Grandfather (as Eugene 'Papaw' Greeson) |
| MMarie GreesonMisty's Grandmother (as Marie 'Mamaw' Greeson) | Misty's Grandmother (as Marie 'Mamaw' Greeson) |
| BBrenda RobinsonFamily Friend and Retired Paralegal | Family Friend and Retired Paralegal |
| PPhilip PatrickJulie's Son | Julie's Son |
| JJohn Bernard(as Dr. John Bernard) | (as Dr. John Bernard) |
| DDavid AlexanderNot listed | Not listed |
| BBrent Polk(as Dr. Brent Polk) | (as Dr. Brent Polk) |
| PPaul HymanPediatric Gastroenterologist | Pediatric Gastroenterologist |
| KKaren CrissingerNot listed | Not listed |
| NNatasha AlexanderNot listed | Not listed |
| BBritney JonesNot listed | Not listed |
| CColin Rudolph(as Dr. Colin Rudolph) | (as Dr. Colin Rudolph) |