 Ben StillerAlex (voice) | Alex (voice) |
 Chris RockMarty / Additional Zebras (voice) | Marty / Additional Zebras (voice) |
 David SchwimmerMelman (voice) | Melman (voice) |
 Jada Pinkett SmithGloria (voice) | Gloria (voice) |
 Sacha Baron CohenJulien (voice) | Julien (voice) |
 Cedric the EntertainerMaurice (voice) | Maurice (voice) |
 Andy RichterMort (voice) | Mort (voice) |
 Bernie MacZuba (voice) | Zuba (voice) |
 Alec BaldwinMakunga (voice) | Makunga (voice) |
 Sherri ShepherdMom (voice) | Mom (voice) |
 will.i.amMoto Moto (voice) | Moto Moto (voice) |
 Elisa GabrielliNana (voice) | Nana (voice) |
 Tom McGrathSkipper / Lemur (voice) | Skipper / Lemur (voice) |
 Chris MillerKowalski (voice) | Kowalski (voice) |
 Christopher KnightsPrivate (voice) | Private (voice) |
 Conrad VernonMason (voice) | Mason (voice) |
| QQuinn Dempsey StillerBaby Alex (voice) | Baby Alex (voice) |
| DDeclan SwiftBaby Alex (voice) | Baby Alex (voice) |
 Fred TatascioreTeetsi / Poacher #1 / Elephant (voice) | Teetsi / Poacher #1 / Elephant (voice) |
 Eric DarnellJoe the Witch Doctor / Poacher #2 (voice) | Joe the Witch Doctor / Poacher #2 (voice) |
 Willow SmithBaby Glorida (voice) | Baby Glorida (voice) |
 Thomas StanleyBaby Marty (voice) | Baby Marty (voice) |
 Zachary GordonBaby Melman (voice) | Baby Melman (voice) |
 Meredith VieiraNewscaster (voice) | Newscaster (voice) |
 Lesley StahlNewscaster (voice) | Newscaster (voice) |
 Al RokerNewscaster (voice) | Newscaster (voice) |
 David SorenLemur (voice) | Lemur (voice) |
 Phil LaMarrGuide (voice) | Guide (voice) |
 Stephen KearinStephen the Giraffe / Tourist with Camera / Rhino (voice) | Stephen the Giraffe / Tourist with Camera / Rhino (voice) |
 Dan O'ConnorTourist with Shirt / Cape Buffalo (voice) | Tourist with Shirt / Cape Buffalo (voice) |
