 Ben StillerAlex (voice) | Alex (voice) |
 Chris RockMarty (voice) | Marty (voice) |
 David SchwimmerMelman (voice) | Melman (voice) |
 Jada Pinkett SmithGloria (voice) | Gloria (voice) |
 Sacha Baron CohenKing Julien (voice) | King Julien (voice) |
 Cedric the EntertainerMaurice (voice) | Maurice (voice) |
 Andy RichterMort (voice) | Mort (voice) |
 Tom McGrathSkipper / Fossa / Panicky Man on Subway (voice) | Skipper / Fossa / Panicky Man on Subway (voice) |
 Christopher KnightsPrivate (voice) | Private (voice) |
 Chris MillerKowalski (voice) | Kowalski (voice) |
 Conrad VernonMason (voice) | Mason (voice) |
 Eric DarnellZoo Announcer / Lemur #1 / Fossa / Subway Car Announcer (voice) | Zoo Announcer / Lemur #1 / Fossa / Subway Car Announcer (voice) |
 David CowgillPolice Horse (voice) | Police Horse (voice) |
 Elisa GabrielliOld Lady (voice) | Old Lady (voice) |
 Stephen ApostolinaPolice Officer (voice) | Police Officer (voice) |
 Devika ParikhNews Reporter (voice) | News Reporter (voice) |
 David P. SmithSpider / Lemur #2 (voice) | Spider / Lemur #2 (voice) |
 Cody CameronWillie (voice) | Willie (voice) |
 Bob SagetZoo Animal (voice) | Zoo Animal (voice) |
 Jeffrey KatzenbergRico (voice) (uncredited) | Rico (voice) (uncredited) |
