When Batman, claiming to be a loner, refuses to join the Justice League, he is drawn into working with them when they are kidnapped one-by-one and held prisoner by one of Batman's nemeses.
| Actor | Role |
|---|---|
 Troy BakerBatman / Bruce Wayne (voice) | Batman / Bruce Wayne (voice) |
 Dee Bradley BakerAquaman / Man-Bat (voice) | Aquaman / Man-Bat (voice) |
 John DiMaggioLex Luthor / Joker (voice) | Lex Luthor / Joker (voice) |
 Grey DeLisleWonder Woman / Lois Lane (voice) | Wonder Woman / Lois Lane (voice) |
 Tom KennyPenguin (voice) | Penguin (voice) |
 Khary PaytonCyborg (voice) | Cyborg (voice) |
 James Arnold TaylorThe Flash (voice) | The Flash (voice) |
 Nolan NorthSuperman / Alfred (voice) | Superman / Alfred (voice) |
 Paul ReubensBat-Mite (voice) | Bat-Mite (voice) |
 Kevin Michael RichardsonCaptain Cold / Black Manta (voice) | Captain Cold / Black Manta (voice) |