When Dr. Frankenstein is killed by a monster he created, his daughter and his lab assistant continue his experiments.
| Actor | Role |
|---|---|
 Joseph CottenBaron Frankenstein | Baron Frankenstein |
 Rosalba NeriTania Frankenstein | Tania Frankenstein |
 Paul MüllerDr. Charles Marshall | Dr. Charles Marshall |
 Herbert FuxTom Lynch | Tom Lynch |
 Renate KaschéJulia Black | Julia Black |
 Mickey HargitayCaptain Harris | Captain Harris |
 Lorenzo TerzonHarris' assistant | Harris' assistant |
 Andrea AureliJim Turner | Jim Turner |
 Ada PomettiSara Willis | Sara Willis |
 Joshua SinclairJohn | John |
| RRichard BeardleySimon Burke | Simon Burke |
| PPetar MartinovitchJack Morgan | Jack Morgan |
 Herb AndressHunchback | Hunchback |
 Marino MaséThomas Stack (as Peter Whiteman) | Thomas Stack (as Peter Whiteman) |
| AAdam WellesChild | Child |
 Riccardo PizzutiThe Creature | The Creature |
 Romano PuppoZack | Zack |