Trixie can't wait to bring Knuffle Bunny to school and show him off. But an awful surprise awaits her: someone else has the exact same bunny! Thus begins an exciting, frustrating and ultimately revelatory twenty-four hours of chaos, where Trixie loses her beloved bunny and gains him back, along with something new: her very first best friend.
| Actor | Role |
|---|---|
| CCher WillemsNarrator / Mommy (voice) | Narrator / Mommy (voice) |
 Mo WillemsNarrator / Daddy (voice) | Narrator / Daddy (voice) |
| TTrixie WillemsNarrator / Trixie (voice) | Narrator / Trixie (voice) |
| SSophie O'BrienSonja (voice) | Sonja (voice) |