In the first feature-length animated movie based on the Hanna-Barbera series, Jonny Quest fans get to meet the women behind the adventurous men. Joining scientist Benton Quest, his plucky son Jonny, bodyguard Race Bannon and Jonny's young pal Hadji are Benton's biologist wife Rachel, Race's ex-wife Jade and young 12-year-old Jessie, who harbors a big secret. Throughout, Team Quest battles the evil schemes of modern-day alchemist Dr. Zin, who has cloned himself and created an army of mutant reptiles in the Peruvian rain forest. The clash there results in a tragedy that changes Jonny's life forever - and later leads to a worldwide pursuit of Zin that includes examining rare Leonardo da Vinci documents in Paris, exploring the Roman catacombs and a final confrontation in the Australian outback.
| Actor | Role |
|---|---|
 Will EstesJonny Quest (voice) | Jonny Quest (voice) |
 Ed GilbertCommander (voice) | Commander (voice) |
 Meredith MacRaeDr. Rachel Quest (voice) | Dr. Rachel Quest (voice) |
 George HearnPresident (voice) | President (voice) |
 Anndi McAfeeJessie (voice) | Jessie (voice) |
| DDon MessickDr. Benton C. Quest / Bandit (voice) | Dr. Benton C. Quest / Bandit (voice) |
 Rob PaulsenHadji (voice) | Hadji (voice) |
 Whit HertfordNot listed | Not listed |
 Pepe SernaAgent Menendez (voice) | Agent Menendez (voice) |
 Peter RenadayDr. Victor Devlon (voice) | Dr. Victor Devlon (voice) |
 Jeffrey TamborDr. Zin (voice) | Dr. Zin (voice) |
 Marcelo TubertChef (voice) | Chef (voice) |
 Granville Van DusenRace Bannon (voice) | Race Bannon (voice) |
 B.J. Ward3-DAC (voice) | 3-DAC (voice) |
 Frank WelkerSnipe / Replicants (voice) | Snipe / Replicants (voice) |