 Debi DerryberryJimmy Neutron / Alien Actress / Alien Woman (voice) | Jimmy Neutron / Alien Actress / Alien Woman (voice) |
 Rob PaulsenCarl Wheezer/Mr. Wheezer/Mrs. Wheezer/Test Pilot/Announcer (voice) | Carl Wheezer/Mr. Wheezer/Mrs. Wheezer/Test Pilot/Announcer (voice) |
 Jeffrey GarciaSheen Estevez / Army Guard (voice) | Sheen Estevez / Army Guard (voice) |
 Frank WelkerGoddard (voice) | Goddard (voice) |
 Carolyn LawrenceCindy Vortex (voice) | Cindy Vortex (voice) |
 Crystal ScalesLibby Folfax (voice) | Libby Folfax (voice) |
 Tim AllenMeldar Prime (voice) | Meldar Prime (voice) |
 Alyssa MilanoApril (voice) | April (voice) |
 Carlos AlazraquiSr. Estevez (voice) | Sr. Estevez (voice) |
 Jeff BennettGorlock #2 / Brain #1 (voice) | Gorlock #2 / Brain #1 (voice) |
 Megan CavanaghJudy Neutron / Mrs. Vortex (voice) | Judy Neutron / Mrs. Vortex (voice) |
 Jim CummingsBrain #2 / Mayor Quadar (voice) | Brain #2 / Mayor Quadar (voice) |
 Mark DeCarloHugh Neutron / Emergency System / Gen. Abercrombie (voice) | Hugh Neutron / Emergency System / Gen. Abercrombie (voice) |
 Jess HarnellMeldar Prime (singing voice) | Meldar Prime (singing voice) |
 Joe LalaSr. Estevez (voice) | Sr. Estevez (voice) |
 Phil LaMarrBolbi Stroganofsky / British Tourist (voice) | Bolbi Stroganofsky / British Tourist (voice) |
 Billy WestSam / Corky Shumatsu (voice) | Sam / Corky Shumatsu (voice) |
 Grey DeLisleVendanna (voice) | Vendanna (voice) |
