 Ray RomanoManny (voice) | Manny (voice) |
 John LeguizamoSid (voice) | Sid (voice) |
 Denis LearyDiego (voice) | Diego (voice) |
 Queen LatifahEllie (voice) | Ellie (voice) |
 Seann William ScottCrash (voice) | Crash (voice) |
 Josh PeckEddie (voice) | Eddie (voice) |
 Jay LenoFast Tony (voice) | Fast Tony (voice) |
 Will ArnettLone Gunslinger Vulture (voice) | Lone Gunslinger Vulture (voice) |
 Chris WedgeScrat (voice) | Scrat (voice) |
 Peter AckermanDung Beetle Dad (voice) | Dung Beetle Dad (voice) |
| CCaitlin Rose AndersonGlypto Boy Billy / Beaver Girl (voice) | Glypto Boy Billy / Beaver Girl (voice) |
| CConnor AndersonRhino Boy / Beaver Boy (voice) | Rhino Boy / Beaver Boy (voice) |
 Joseph BolognaMr. Start (voice) | Mr. Start (voice) |
| JJack CrocicchiaElk Boy (voice) | Elk Boy (voice) |
 Peter de SèveCondor Chick (voice) | Condor Chick (voice) |
 Ariel WinterAdditional Voices (voice) | Additional Voices (voice) |
 Clea LewisFemale Mini Sloth (voice) | Female Mini Sloth (voice) |
 Stephen RootAardvark Dad (voice) | Aardvark Dad (voice) |
| NNicole DeFeliceStart Girl (voice) | Start Girl (voice) |
 Debi DerryberryDiatryma Mom (voice) | Diatryma Mom (voice) |
 Marshall EfronStart Dad (voice) | Start Dad (voice) |
 Tom FahnStu / Male Ox (voice) | Stu / Male Ox (voice) |
 Jason FricchioneMolehog Grandpa / Shovelmouth Male (voice) | Molehog Grandpa / Shovelmouth Male (voice) |
| GGeorge JacobsBeaver Dad (voice) | Beaver Dad (voice) |
 Brian Scott McFaddenGlyptodon (voice) | Glyptodon (voice) |
 Jansen PanettiereShovelmouth Boy (voice) | Shovelmouth Boy (voice) |
 Gregory RomanoStart Boy 1 (voice) | Start Boy 1 (voice) |
 Matt RomanoStart Boy 2 (voice) | Start Boy 2 (voice) |
 Carlos SaldanhaDodo (voice) | Dodo (voice) |
| MManoela Scarpa SaldanhaMolehog Kid 1 (voice) | Molehog Kid 1 (voice) |
