 Ray RomanoManny (voice) | Manny (voice) |
 John LeguizamoSid (voice) | Sid (voice) |
 Denis LearyDiego (voice) | Diego (voice) |
 Seann William ScottCrash (voice) | Crash (voice) |
 Josh PeckEddie (voice) | Eddie (voice) |
 Peter DinklageCaptain Gutt (voice) | Captain Gutt (voice) |
 Wanda SykesGranny (voice) | Granny (voice) |
 Jennifer LopezShira (voice) | Shira (voice) |
 Queen LatifahEllie (voice) | Ellie (voice) |
 Keke PalmerPeaches (voice) | Peaches (voice) |
 Josh GadLouis (voice) | Louis (voice) |
 Nick FrostFlynn (voice) | Flynn (voice) |
 Aziz AnsariSquint (voice) | Squint (voice) |
 DrakeEthan (voice) | Ethan (voice) |
 Nicki MinajSteffie (voice) | Steffie (voice) |
 Alan TudykMilton / Hunky Siren (voice) | Milton / Hunky Siren (voice) |
 Ester DeanFemale Siren (voice) | Female Siren (voice) |
 Kunal NayyarGupta (voice) | Gupta (voice) |
 Rebel WilsonRaz (voice) | Raz (voice) |
 Eddie 'Piolin' SoteloUncle Fungus (voice) | Uncle Fungus (voice) |
 Joy BeharEunice (voice) | Eunice (voice) |
 Alain ChabatSilas (voice) | Silas (voice) |
 Heather MorrisKatie (voice) | Katie (voice) |
 Chris WedgeScrat (voice) | Scrat (voice) |
 Karen DisherScratte (voice) | Scratte (voice) |
| CChristopher CampbellCreature Siren (voice) | Creature Siren (voice) |
 Marieve HeringtonAdditional Voices (voice) | Additional Voices (voice) |
| LLucas LeguizamoAdditional Voices (voice) | Additional Voices (voice) |
 Allegra LeguizamoAdditional Voices (voice) | Additional Voices (voice) |
 Jason FricchioneDumb Mammoth / Various (voice) | Dumb Mammoth / Various (voice) |
