 Assumpta SernaJuana Inés de la Cruz | Juana Inés de la Cruz |
 Dominique SandaLa Virreina | La Virreina |
 Héctor AlterioEl Virrey | El Virrey |
 Lautaro MurúaArzobispo | Arzobispo |
 Alberto SegadoPadre Miranda | Padre Miranda |
| FFranklin CaicedoSanta Cruz | Santa Cruz |
 Graciela AraujoHermana Ursula | Hermana Ursula |
 Hugo SotoAmante en flashback del palacio | Amante en flashback del palacio |
 Márgara AlonsoNot listed | Not listed |
 Lidia CatalanoMadre de Juana | Madre de Juana |
| MMargarita PadínNot listed | Not listed |
 Gerardo RomanoSigüenza | Sigüenza |
 Cecilia RothLa Virreina (voice) | La Virreina (voice) |
| AAlejandra ColungaNot listed | Not listed |
 Rosario BléfariNot listed | Not listed |
| FFelisa RochaNot listed | Not listed |
| EEma RiveraNot listed | Not listed |
| FFernando NoyNot listed | Not listed |
| JJosé MartinNot listed | Not listed |
 Walter SoubriéNot listed | Not listed |
 Jesús BerenguerNot listed | Not listed |
 Guillermo MarínNot listed | Not listed |
| NNicolás AlvaradoNot listed | Not listed |
| SSusana CortínezNot listed | Not listed |
| AAlice EliasNot listed | Not listed |
| MMónica LacosteNot listed | Not listed |
| PPedro CañaNot listed | Not listed |
 Marcos WoinskyMiembro auditoria de textos de Juana | Miembro auditoria de textos de Juana |
| IIsaac HaimoviciNot listed | Not listed |
| NNéstor ZaccoNot listed | Not listed |
