 Sid HaigCaptain Spaulding | Captain Spaulding |
 Bill MoseleyOtis B. Driftwood | Otis B. Driftwood |
 Sheri Moon ZombieBaby Firefly | Baby Firefly |
 Karen BlackMother Firefly | Mother Firefly |
 Erin DanielsDenise Willis | Denise Willis |
 Chris HardwickJerry Goldsmith | Jerry Goldsmith |
 Rainn WilsonBill Hudley | Bill Hudley |
 Jennifer JostynMary Knowles | Mary Knowles |
 Tom TowlesLieutenant George Wydell | Lieutenant George Wydell |
 Walton GogginsDeputy Steve Naish | Deputy Steve Naish |
 Matthew McGroryTiny Firefly | Tiny Firefly |
 Robert Allen MukesRufus 'RJ' Firefly Jr. | Rufus 'RJ' Firefly Jr. |
 Dennis FimpleGrandpa Hugo Firefly | Grandpa Hugo Firefly |
 Jake McKinnonRufus 'Earl' Firefly Sr. | Rufus 'Earl' Firefly Sr. |
 Harrison YoungDon Willis | Don Willis |
 Irwin KeyesRavelli | Ravelli |
 Michael J. PollardStucky | Stucky |
 Chad BannonKiller Karl | Killer Karl |
 William BassettSheriff Frank Huston | Sheriff Frank Huston |
 David ReynoldsRichard 'Little Dick' Wick | Richard 'Little Dick' Wick |
| JJoe Dobbs IIIGerry Ober | Gerry Ober |
 Judith DrakeSkunk Ape Wife | Skunk Ape Wife |
| GGregg GibbsDr. Wolfenstein | Dr. Wolfenstein |
| KKen JohnsonSkunk Ape Husband | Skunk Ape Husband |
| IIrvin Mosley Jr.Lewis Dover | Lewis Dover |
| Tom TowlesGeorge Wydell | George Wydell |
 Walter PhelanDr. Satan | Dr. Satan |
 Rob ZombieDr. Wolfenstein's assistant (uncredited) | Dr. Wolfenstein's assistant (uncredited) |
