Scooby-Doo and the gang team up with their pals, Bill Nye The Science Guy and Elvira Mistress of the Dark, to solve this mystery of gigantic proportions and save Crystal Cove!
| Actor | Role |
|---|---|
 Frank WelkerScooby-Doo / Fred Jones (voice) | Scooby-Doo / Fred Jones (voice) |
 Grey DeLisleDaphne Blake (voice) | Daphne Blake (voice) |
 Matthew LillardShaggy Rogers (voice) | Shaggy Rogers (voice) |
 Kate MicucciVelma Dinkley (voice) | Velma Dinkley (voice) |
 Cassandra PetersonElvira, Mistress of the Dark (voice) | Elvira, Mistress of the Dark (voice) |
 Bill NyeBill Nye (voice) | Bill Nye (voice) |
 Dwight SchultzDr. Crane, AKA: Scarecrow (voice) | Dr. Crane, AKA: Scarecrow (voice) |
 Kamali MinterMichelle (voice) | Michelle (voice) |
 Bumper RobinsonMike (voice) | Mike (voice) |
 Roger Craig SmithAutopilot / Parent (voice) | Autopilot / Parent (voice) |
 Fred TatascioreAlpha Jackal-Lantern / Federal Agent Malarkey (voice) | Alpha Jackal-Lantern / Federal Agent Malarkey (voice) |
 David HermanSheriff, AKA: Cutler Toe (voice) | Sheriff, AKA: Cutler Toe (voice) |