Upon meeting a young boy named Seita who makes a living as a pickpocket, Gintoki discovers that he is pickpocketing out of a desire to meet his long-lost mother, Hinowa, Yoshiwara's number one courtesan. Gintoki and his friends venture to Yoshiwara to fulfill Seita's wish, but standing in their way is Housen, the Night King who rules Yoshiwara.
| Actor | Role |
|---|---|
 Tomokazu SugitaGintoki Sakata (voice) | Gintoki Sakata (voice) |
 Daisuke SakaguchiShinpachi Shimura (voice) | Shinpachi Shimura (voice) |
 Rie KugimiyaKagura (voice) | Kagura (voice) |
 Satoshi HinoKamui (voice) | Kamui (voice) |
 Yuhko KaidaTsukuyo (voice) | Tsukuyo (voice) |
 Banjo GingaHousen (voice) | Housen (voice) |
 Yuko SanpeiSeita (voice) | Seita (voice) |
 Kikuko InoueHinowa (voice) | Hinowa (voice) |
 Hochu OtsukaAbuto (voice) | Abuto (voice) |
 Kappei YamaguchiInorin (voice) | Inorin (voice) |
 Akira IshidaKotaro Katsura (voice) | Kotaro Katsura (voice) |
 Susumu ChibaIsao Kondo (voice) | Isao Kondo (voice) |
 Kazuya NakaiToshiro Hijikata (voice) | Toshiro Hijikata (voice) |
 Kenichi SuzumuraOkita Sogo (voice) | Okita Sogo (voice) |
 Tetsuharu OtaSagaru Yamazaki (voice) | Sagaru Yamazaki (voice) |
 KujiraOtose (voice) | Otose (voice) |
 Yuu SugimotoCatherine (voice) | Catherine (voice) |
 Omi MinamiTama (voice) | Tama (voice) |