In the future, one can achieve immortality by obtaining a mechanized body. Orphaned, young Tetsuro hitches a ride on the space train Galaxy Express 999 in the hope of obtaining a cyborg body to avenge his mother's death. Along the way, he meets Maetel, who is the spitting image of his dead mother.
| Actor | Role |
|---|---|
 Masako NozawaTetsurô Hoshino (voice) | Tetsurô Hoshino (voice) |
 Masako IkedaMaetel (voice) | Maetel (voice) |
 Yoko AsagamiClaire (voice) | Claire (voice) |
 Miyoko AsoTochirô's Mother (voice) | Tochirô's Mother (voice) |
 Toshiko FujitaShadow (voice) | Shadow (voice) |
 Yasuo HisamatsuAntares (voice) | Antares (voice) |
 Makio InoueCaptain Harlock (voice) | Captain Harlock (voice) |
| TTatsuya JoNarrator (voice) | Narrator (voice) |
 Ryoko KinomiyaQueen Promethium (voice) | Queen Promethium (voice) |
 Kaneta KimotsukiConductor (voice) | Conductor (voice) |
 Gorō NayaDoctor Ban (voice) | Doctor Ban (voice) |
 Noriko OharaRyûzu / Mîmé (voice) | Ryûzu / Mîmé (voice) |
 Banjo GingaCaptain of the Guard (voice) | Captain of the Guard (voice) |
 Kei TomiyamaTochirô Ôyama (voice) | Tochirô Ôyama (voice) |
 Reiko TajimaEmeraldas (voice) | Emeraldas (voice) |
 Hidekatsu ShibataCount Mecha (voice) | Count Mecha (voice) |
 Akiko TsuboiKanae Hoshino (Tetsurô's mother) (voice) | Kanae Hoshino (Tetsurô's mother) (voice) |
 Ryuji SaikachiTavern Owner (voice) | Tavern Owner (voice) |