 Colin CliveHenry Frankenstein | Henry Frankenstein |
 Mae ClarkeElizabeth | Elizabeth |
 John BolesVictor Moritz | Victor Moritz |
 Boris KarloffThe Monster | The Monster |
 Edward Van SloanDoctor Waldman | Doctor Waldman |
 Frederick KerrBaron Frankenstein | Baron Frankenstein |
 Dwight FryeFritz | Fritz |
 Lionel BelmoreThe Burgomaster | The Burgomaster |
 Marilyn HarrisLittle Maria | Little Maria |
 Ted BillingsVillager (uncredited) | Villager (uncredited) |
| MMae BruceScreaming Maid (uncredited) | Screaming Maid (uncredited) |
| JJack CurtisVillager (uncredited) | Villager (uncredited) |
 Arletta DuncanBridesmaid (uncredited) | Bridesmaid (uncredited) |
 William DyerGravedigger (uncredited) | Gravedigger (uncredited) |
 Francis FordHans (uncredited) | Hans (uncredited) |
 Soledad JiménezMourner (uncredited) | Mourner (uncredited) |
 Carmencita JohnsonLittle Girl (uncredited) | Little Girl (uncredited) |
| SSeessel Anne JohnsonLittle Girl (uncredited) | Little Girl (uncredited) |
 Margaret MannMourner (uncredited) | Mourner (uncredited) |
 Michael MarkLudwig (uncredited) | Ludwig (uncredited) |
 Pauline MooreBridesmaid (uncredited) | Bridesmaid (uncredited) |
 Inez PalangeVillager (uncredited) | Villager (uncredited) |
 Paul PanzerMourner (uncredited) | Mourner (uncredited) |
 Cecilia ParkerMaid (uncredited) | Maid (uncredited) |
| RRose PlumerVillager (uncredited) | Villager (uncredited) |
| CCecil ReynoldsWaldman's Secretary (uncredited) | Waldman's Secretary (uncredited) |
 Ellinor VanderveerMedical Student (uncredited) | Medical Student (uncredited) |
 Robert MilaschVillager (uncredited) | Villager (uncredited) |
