In Osaka during the Edo period, ruler Sanada Yukimura was in losing position, fearing Toyotomi's blood descendents would end, he impregnates one of five Shinano female ninja with the seed of Toyotomi Hideyoshi in hopes of continuing the bloodline. Princess Sen and the five female ninja escape from Osaka castle by blending in with the handmaidens. Lord Tokugawa Ieyasu who was supposed to rule all under the heavens was informed of this secret. So Ieyasu orders five samurai of the Iga Ninja clan (brought by Hattori Hanzo) to stop the Toyotomi bloodline by killing the Kunoichi ninja. An attack of ninja magic arts unfolds in a battle of the sexes.
| Actor | Role |
|---|---|
 Yumiko NogawaPrincess Sen | Princess Sen |
 Sanae NakaharaOyui | Oyui |
 Yuriko MishimaOyo | Oyo |
 Mari YoshimuraOmayu | Omayu |
 Kyōsuke MachidaSutebei | Sutebei |
 Shingo YamashiroAmamaki | Amamaki |
 Ryūji ShinagawaHattori Hanzo | Hattori Hanzo |
 Shoichi OzawaUsuzumi | Usuzumi |
 Shigeru TsuyuguchiAkiaki Sakazaki | Akiaki Sakazaki |
 Ryōsuke KagawaNot listed | Not listed |
 Yoshio YoshidaHannyadera Fuhaku | Hannyadera Fuhaku |
 Eizō KitamuraNot listed | Not listed |
| KKokingo IchikawaNot listed | Not listed |
| MMitsuko AoiOnami | Onami |
| KKatsumi KanekoTakashi | Takashi |
| KKeiko KojimaNot listed | Not listed |
| MMiyuki SasaNot listed | Not listed |
| AAyako SatōNot listed | Not listed |
| MMinako YamanoNot listed | Not listed |
| AAkiko MatsushiroNot listed | Not listed |
 Kinji NakamuraNot listed | Not listed |
 Yoshihiro IgarashiNot listed | Not listed |
| KKinnosuke TakamatsuNot listed | Not listed |
| HHiroshi HatanoNot listed | Not listed |
| TTakashi KajiNot listed | Not listed |
| RRyōta MinowadaNot listed | Not listed |
 Meichō SoganoyaIeyasu Tokugawa | Ieyasu Tokugawa |
 Michiyo KogureAfuku | Afuku |
 Minoru ŌkiTsuzumi Hayato | Tsuzumi Hayato |