Shirou has lost his Servant, Saber, and is no longer a Master in the Holy Grail War. Despite this, he refuses to leave the battle, determined to protect Sakura. Meanwhile, a mysterious shadow engulfs the city, killing Masters and Servants one by one. As Shirou faces new dangers, Sakura is drawn deeper into her own destiny as a mage.
| Actor | Role |
|---|---|
 Noriaki SugiyamaShirou Emiya (voice) | Shirou Emiya (voice) |
 Noriko ShitayaSakura Matou (voice) | Sakura Matou (voice) |
 Hiroshi KamiyaShinji Matou (voice) | Shinji Matou (voice) |
 Ayako KawasumiSaber Alter (voice) | Saber Alter (voice) |
 Kana UedaRin Tohsaka (voice) | Rin Tohsaka (voice) |
 Miki ItoTaiga Fujimura (voice) | Taiga Fujimura (voice) |
 Jouji NakataKirei Kotomine (voice) | Kirei Kotomine (voice) |
 Masane TsukayamaZouken Matou (voice) | Zouken Matou (voice) |
 Tomokazu SekiGilgamesh (voice) | Gilgamesh (voice) |
 Yuu AsakawaRider (voice) | Rider (voice) |
 Tetsu InadaTrue Assassin (voice) | True Assassin (voice) |
 Junichi SuwabeArcher (voice) | Archer (voice) |
 Mai KadowakiIllyasviel von Einzbern (voice) | Illyasviel von Einzbern (voice) |
 Shinya Takahashi(voice) | (voice) |
 Ryuichi Kijima(voice) | (voice) |
 Riho Sugiyama(voice) | (voice) |
 Ayumi Mano(voice) | (voice) |
 Chiaki Kobayashi(voice) | (voice) |
 Aoi Koga(voice) | (voice) |
| KKaoruko Negishi(voice) | (voice) |