The Yamato and her crew face the onslaught of the Comet Empire, a civilization from the Andromeda Galaxy who seek to conquer Earth, led by Prince Zordar. They have harnessed a comet and is using its terrible power to unleash destruction upon its rivals and inferiors... which are in fact everyone. The Space Battleship Yamato heads back into action. But this time, it shall not return, and much of the Yamato crew have signed on for their last voyage.
| Actor | Role |
|---|---|
 Kei TomiyamaSusumu Kodai (voice) | Susumu Kodai (voice) |
 Yoko AsagamiYuki Mori (voice) | Yuki Mori (voice) |
 Gorō NayaJuzo Okita (voice) | Juzo Okita (voice) |
 Shūsei NakamuraDaisuke Shima (voice) | Daisuke Shima (voice) |
 Kenichi OgataAnalyzer (voice) | Analyzer (voice) |
 Shinji NomuraYoshikazu Aihara (voice) | Yoshikazu Aihara (voice) |
 Yoshito YasuharaKenjiro Ota / Politician C (voice) | Kenjiro Ota / Politician C (voice) |
 Akira KamiyaSaburo Kato (voice) | Saburo Kato (voice) |
 Kazuo HayashiYasuo Nanbu (voice) | Yasuo Nanbu (voice) |
 Kazuyuki SogabeAkira Yamamoto / Razelar (voice) | Akira Yamamoto / Razelar (voice) |
| AAkira KimuraRyû Hijikata (voice) | Ryû Hijikata (voice) |
 Isao SasakiHajime Saito (voice) | Hajime Saito (voice) |
 Ichiro NagaiSakezo Sado / Hikozaemon Tokugawa (voice) | Sakezo Sado / Hikozaemon Tokugawa (voice) |
 Takeshi AonoShiro Sanada (voice) | Shiro Sanada (voice) |