Under constant attack by monstrous creatures called Angels that seek to eradicate humankind, U.N. Special Agency NERV introduces two new EVA pilots to help defend the city of Tokyo-3: the mysterious Makinami Mari Illustrous and the intense Asuka Langley Shikinami. Meanwhile, Gendo Ikari and SEELE proceed with a secret project that involves both Rei and Shinji.
| Actor | Role |
|---|---|
 Megumi OgataShinji Ikari (voice) | Shinji Ikari (voice) |
 Megumi HayashibaraRei Ayanami / Yui Ikari (voice) | Rei Ayanami / Yui Ikari (voice) |
 Yuko MiyamuraAsuka Shikinami Langley (voice) | Asuka Shikinami Langley (voice) |
 Maaya SakamotoMari Makinami Illustrious (voice) | Mari Makinami Illustrious (voice) |
 Kotono MitsuishiMisato Katsuragi (voice) | Misato Katsuragi (voice) |
 Yuriko YamaguchiRitsuko Akagi (voice) | Ritsuko Akagi (voice) |
 Koichi YamaderaRyoji Kaji (voice) | Ryoji Kaji (voice) |
 Fumihiko TachikiGendo Ikari (voice) | Gendo Ikari (voice) |
 Motomu KiyokawaKozo Fuyutsuki (voice) | Kozo Fuyutsuki (voice) |
 Miki NagasawaMaya Ibuki (voice) | Maya Ibuki (voice) |
 Takehito KoyasuShigeru Aoba (voice) | Shigeru Aoba (voice) |
 Hiro YuukiMakoto Hyuuga (voice) | Makoto Hyuuga (voice) |
 Tomokazu SekiToji Suzuhara (voice) | Toji Suzuhara (voice) |
 Tetsuya IwanagaKensuke Aida (voice) | Kensuke Aida (voice) |
 Junko IwaoHikari Horaki (voice) | Hikari Horaki (voice) |
 MugihitoKeel Lorenz (voice) | Keel Lorenz (voice) |