 Cate BlanchettElizabeth I, Queen of England | Elizabeth I, Queen of England |
 Laurence FoxSir Christopher Hatton | Sir Christopher Hatton |
 Clive OwenSir Walter Raleigh | Sir Walter Raleigh |
 Geoffrey RushSir Francis Walsingham | Sir Francis Walsingham |
 Tom HollanderAmyas Paulet | Amyas Paulet |
 Abbie CornishElizabeth Throckmorton | Elizabeth Throckmorton |
 Rhys IfansRobert Reston | Robert Reston |
 Samantha MortonMary, Queen of Scots | Mary, Queen of Scots |
 Jordi MollàKing Philip II of Spain | King Philip II of Spain |
 Eddie RedmayneAnthony Babington | Anthony Babington |
 Adrian ScarboroughCalley | Calley |
 Adam GodleyWilliam Walsingham | William Walsingham |
| RRobert CambrinusCount Georg von Helfenstein | Count Georg von Helfenstein |
 Christian BrassingtonArchduke Charles | Archduke Charles |
 David ThrelfallDr. John Dee | Dr. John Dee |
 Vidal SanchoSpanish Minister | Spanish Minister |
 John ShrapnelLord Howard | Lord Howard |
 Sam SpruellTorturer | Torturer |
 Kelly HunterUrsula Walsingham | Ursula Walsingham |
 David SterneCellarman | Cellarman |
 David RobbAdmiral Sir William Winter | Admiral Sir William Winter |
 Jonathan BaileyCourtier | Courtier |
| SSteve LatelyWalsingham's Servant | Walsingham's Servant |
 Kate FleetwoodWoman with Baby | Woman with Baby |
| AAimee KingInfanta Isabel of Spain | Infanta Isabel of Spain |
 Susan LynchAnnette | Annette |
| KKristin Coulter SmithMary Walsingham | Mary Walsingham |
 Hayley BurroughsQueen Elizabeth's Waiting Lady #1 | Queen Elizabeth's Waiting Lady #1 |
| KKirsty McKayQueen Elizabeth's Waiting Lady #2 | Queen Elizabeth's Waiting Lady #2 |
| LLucia Ruck KeeneQueen Elizabeth's Waiting Lady #3 | Queen Elizabeth's Waiting Lady #3 |
