A shy comic book fan is injected with an experimental serum and starts turning into a spider. When web covered bodies start appearing a policeman starts to investigate the strange case.
| Actor | Role |
|---|---|
 Dan AykroydDet. Insp. Jack Grillo | Det. Insp. Jack Grillo |
 Devon GummersallQuentin Kemmer | Quentin Kemmer |
 Amelia HeinleStephanie Lewis | Stephanie Lewis |
 Theresa RussellTrixie Grillo | Trixie Grillo |
 Christopher CousinsOfficer Williams | Officer Williams |
 Mario RoccuzzoNick Bezis | Nick Bezis |
 John ChoHan | Han |
 Randall HuberMidtown murderer | Midtown murderer |
 Zia HarrisGutterpunk #1 | Gutterpunk #1 |
| LLloyd Lowe Jr.Gutterpunk #2 | Gutterpunk #2 |
 Michael KeenanWillie | Willie |
 Ted RooneyCoroner | Coroner |
 Dan MartinHuge Cop | Huge Cop |
| RRob HillLiquor Store Cop | Liquor Store Cop |
| BBrian J. GilbertPhotojournalist | Photojournalist |
| HHoward GeorgeWalter | Walter |
| DDouglas Jude McKeonFacility Manager | Facility Manager |
 Lisa PicotteGoth Girl | Goth Girl |
 Pedro PascalGoth Guy | Goth Guy |
 Willie C. CarpenterDr. Calloway | Dr. Calloway |
| JJessica Littlefield'50s Girl (uncredited) | '50s Girl (uncredited) |