In Earth Defense Girl Iko-chan 2: Lunna's Secret, the mantle of Earth Defense Girl is passed to a new hero after the retirement of Iko Kawai. The new Iko-chan must protect Earth from a new threat, all while navigating the chaos of battling monsters and saving the day.
| Actor | Role |
|---|---|
 Mia MasudaNot listed | Not listed |
| MMamiko TayamaNot listed | Not listed |
 Naoto TakenakaNot listed | Not listed |