In the wake of two back-to-back mass murders on Chico's frat row, loner Brent Chirino must infiltrate the ranks of a popular fraternity to investigate his twin brother's murder at the hands of the serial killer known as "Motherface."
| Actor | Role |
|---|---|
 Alec OwenBrock/Brent | Brock/Brent |
 Patton OswaltChief | Chief |
 Paul PradoTurbeaux | Turbeaux |
 Olivia Taylor DudleyMotherface | Motherface |
 Nina HartleyDean Pepperstone | Dean Pepperstone |
 Greg SesteroDerek | Derek |
| BBen GigliSamzy | Samzy |
| JJoey ScomaTodd | Todd |
 Kelsey GunnSamantha | Samantha |
 Brian FirenziOfficer Sminkle | Officer Sminkle |
 Maria Del CarmenCandace Buttiker | Candace Buttiker |
| MMike JamesZ.Q. | Z.Q. |
 Jimmy WongSizzler | Sizzler |
 Michael RousseletSpike | Spike |
 Nick KocherFlannel Bro | Flannel Bro |
 Brian McElhaneyTurtleneck Bro | Turtleneck Bro |
 Andrew W.K.Rip Stick | Rip Stick |
 Larry KingCoach Handsey | Coach Handsey |
| TTomm JacobsenDolphman | Dolphman |
| EErik SandovalRoad Doggie | Road Doggie |
| CChelsea St. JohnC-Trunk | C-Trunk |
 Christiann CastellanosLady #1 | Lady #1 |
| SSkyler RousseletCindy | Cindy |
 Maura MurphyHot Chick | Hot Chick |
| LLogan OlsonManscaping Bro | Manscaping Bro |
| RRocky CollinsParty Bro | Party Bro |
| JJon WorleyScooter | Scooter |
 Matt HargreavesAsian Bro | Asian Bro |
| IIan AhernDrunk Bro | Drunk Bro |
| LLaura CebrickTri Beta Streakers | Tri Beta Streakers |