 Basil RathboneSherlock Holmes | Sherlock Holmes |
 Nigel BruceDr. John H. Watson | Dr. John H. Watson |
 Patricia MorisonMrs. Hilda Courtney | Mrs. Hilda Courtney |
 Frederick WorlockColonel Cavanaugh | Colonel Cavanaugh |
 Harry CordingHamid | Hamid |
 Carl HarbordInspector Hopkins | Inspector Hopkins |
 Holmes HerbertEbenezer Crabtree | Ebenezer Crabtree |
 Edmund BreonJulian 'Stinky' Emery | Julian 'Stinky' Emery |
| PPatricia CameronEvelyn Clifford | Evelyn Clifford |
 Mary GordonMrs. Hudson | Mrs. Hudson |
 Ian WolfeCommissioner of Scotland Yard | Commissioner of Scotland Yard |
 Leyland HodgsonTour Guide | Tour Guide |
 Marjorie BennettAntique Shop Assistant (uncredited) | Antique Shop Assistant (uncredited) |
 William H. O'BrienDetective at Murder Scene | Detective at Murder Scene |
 Frank BakerPhotographer | Photographer |
 Sally ShepherdNot listed | Not listed |
| WWallace ScottJoe Cisto | Joe Cisto |
| JJack CurtisPub Patron (uncredited) | Pub Patron (uncredited) |
 Cyril DelevantiConvict at Dartmoor Prison (uncredited) | Convict at Dartmoor Prison (uncredited) |
 Charlie HallCab Driver (uncredited) | Cab Driver (uncredited) |
 Olaf HyttenAlfred - Auction House Bookkeeper (uncredited) | Alfred - Auction House Bookkeeper (uncredited) |
 Frank MillsPub Patron (uncredited) | Pub Patron (uncredited) |
 Lillian BronsonMinister's Wife (uncredited) | Minister's Wife (uncredited) |
| BBobby HalePub Patron (uncredited) | Pub Patron (uncredited) |
| AAlexander PollardCrabtree's Assistant at Auction (uncredited) | Crabtree's Assistant at Auction (uncredited) |
