 Sean ConneryJames Bond | James Bond |
 Ursula AndressHoney Ryder | Honey Ryder |
 Joseph WisemanDr. No | Dr. No |
 Jack LordFelix Leiter | Felix Leiter |
 Anthony DawsonProfessor R. J. Dent | Professor R. J. Dent |
 Zena MarshallMiss Taro | Miss Taro |
 John KitzmillerQuarrel | Quarrel |
 Eunice GaysonSylvia Trench | Sylvia Trench |
 Bernard LeeM | M |
 Lois MaxwellMiss Moneypenny | Miss Moneypenny |
 Peter BurtonMajor Boothroyd | Major Boothroyd |
 Yvonne ShimaSister Lily | Sister Lily |
 Michel MokSister Rose | Sister Rose |
 Marguerite LeWarsAnnabel Chung - Photographer | Annabel Chung - Photographer |
 William Foster-DavisSuperintendent Duff | Superintendent Duff |
 Dolores KeatorMary Trueblood | Mary Trueblood |
 Reginald CarterMr. Jones | Mr. Jones |
| LLouis BlaazerPleydell-Smith | Pleydell-Smith |
 Colonel BurtonGeneral Potter | General Potter |
| JJohn HattonSignals Foreman (uncredited) | Signals Foreman (uncredited) |
| BByron LeeBandleader (uncredited) | Bandleader (uncredited) |
| TTimothy Napier MoxonProfessor John Strangways (uncredited) | Professor John Strangways (uncredited) |
| MMalou PanteraHotel Receptionist (uncredited) | Hotel Receptionist (uncredited) |
 Diana CouplandHoney Ryder - Singing Voice (voice) (uncredited) | Honey Ryder - Singing Voice (voice) (uncredited) |
 Nikki Van der ZylHoney Ryder / Sylvia Trench / Various (voice) (uncredited) | Honey Ryder / Sylvia Trench / Various (voice) (uncredited) |
| AAdrian RobinsonHearse Driver (uncredited) | Hearse Driver (uncredited) |
 Bob SimmonsJames Bond in Gunbarrel Sequence (uncredited) | James Bond in Gunbarrel Sequence (uncredited) |
 Milton ReidDr. No's Guard (uncredited) | Dr. No's Guard (uncredited) |
| PPeter EvansLe Cercle Patron (uncredited) | Le Cercle Patron (uncredited) |
| LLester PendergastPuss-Feller (uncredited) | Puss-Feller (uncredited) |
