 Spencer TracyDr. Henry 'Harry' Jekyll / Mr. Hyde | Dr. Henry 'Harry' Jekyll / Mr. Hyde |
 Ingrid BergmanIvy Peterson | Ivy Peterson |
 Lana TurnerBeatrix 'Bea' Emery | Beatrix 'Bea' Emery |
 Donald CrispSir Charles Emery | Sir Charles Emery |
 Ian HunterDr. John Lanyon | Dr. John Lanyon |
 Barton MacLaneSam Higgins | Sam Higgins |
 C. Aubrey SmithThe Bishop | The Bishop |
 Peter GodfreyPoole | Poole |
 Sara AllgoodMrs. Higgins | Mrs. Higgins |
 Frederick WorlockDr. Heath | Dr. Heath |
 William TannenIntern Fenwick | Intern Fenwick |
 Frances RobinsonMarcia | Marcia |
 Denis GreeneFreddie | Freddie |
 Billy BevanMr. Weller | Mr. Weller |
 Forrester HarveyOld Prouty | Old Prouty |
 Lumsden HareColonel Weymouth | Colonel Weymouth |
 Lawrence GrantDr. Courtland | Dr. Courtland |
 John BarclayConstable | Constable |
 Hillary BrookeMrs. Arnold (uncredited) | Mrs. Arnold (uncredited) |
 Alec CraigTripped Waiter (uncredited) | Tripped Waiter (uncredited) |
 Brandon HurstDr. Lanyon's Butler Briggs (uncredited) | Dr. Lanyon's Butler Briggs (uncredited) |
 Olaf HyttenHobson (uncredited) | Hobson (uncredited) |
 Colin KennyConstable (uncredited) | Constable (uncredited) |
 Doris LloydMrs. Marley (uncredited) | Mrs. Marley (uncredited) |
 Aubrey MatherInspector (uncredited) | Inspector (uncredited) |
 Al FergusonConstable (uncredited) | Constable (uncredited) |
| MMary FieldWife (uncredited) | Wife (uncredited) |
 Frank HagneyDrunk (uncredited) | Drunk (uncredited) |
| BBobby HaleCart Driver (uncredited) | Cart Driver (uncredited) |
 Martha WentworthLandlady (uncredited) | Landlady (uncredited) |
