The three brothers discover that the human experiment banned after destroying the "Orochi", is still going on to secretly revive the evil snake. They also find out that some ex-members of the Yamainu (the Japanese military unit formed to kill the snake) are behind this conspiracy. The brothers fight together again in a battle against the military.
| Actor | Role |
|---|---|
 Yuki KajiSoramaru Kumo (voice) | Soramaru Kumo (voice) |
 Yuichi NakamuraTenka Kumo (voice) | Tenka Kumo (voice) |
 Tsubasa YonagaChutaro Kumo (voice) | Chutaro Kumo (voice) |
 Mamiko NotoNishiki (voice) | Nishiki (voice) |
 Kohsuke ToriumiSosei Abeno (voice) | Sosei Abeno (voice) |
 Hiroki YasumotoSeiichiro Takamine (voice) | Seiichiro Takamine (voice) |
 Hiro ShimonoRakucho Takeda (voice) | Rakucho Takeda (voice) |
 Sayaka OharaKiiko Sasaki (voice) | Kiiko Sasaki (voice) |
 Fujiwara YukiMutsuki Ashiya (voice) | Mutsuki Ashiya (voice) |
 Takahiro FujiwaraZenzo Inukai (voice) | Zenzo Inukai (voice) |
 Hiroshi IwasakiChenran Shi (voice) | Chenran Shi (voice) |